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Next-Generation Sequencing Informatics, Second Edition

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undefinedNext-generation sequencing (NGS) technology has revolutionized biomedical research, making genome and RNA sequencing an affordable and frequently used tool for a wide variety of research applications including variant (mutation) discovery, gene expression, transcription factor analysis, metagenomics, and epigenetics. Bioinformatics methods to support sequencing have become and remain a critical bottleneck for many researchers and organizations wishing to make use of NGS technology. This new edition provides a thorough, plain-language introduction to the necessary informatics methods and tools for analyzing NGS data and provides detailed descriptions of algorithms, strengths and weaknesses of specific tools, pitfalls, and alternative methods. Four new chapters cover experimental design, sample preparation, and quality assessment of NGS data; public databases for sequencing data; de novo transcript assembly; proteogenomics; and emerging sequencing technologies. The remaining chapters from the first edition have been updated with the latest information. This book also provides extensive reference to best-practice bioinformatics methods for NGS applications and tutorials for common workflows. This edition addresses the informatics needs of students, laboratory scientists, and computing spets who wish to take advantage of the explosion of research rtunities offered by new sequencing technologies. Authors Alexander Alekseyenko Silvia Argimón Brian D. Athey Miroslav Blumenberg Stuart M. Brown Lisa Cohen Efstratios Efstathiadis David Fenyö Jeremy Goecks Gerald A. Higgins D. Frank Hsu Kranti Konganti Konstantinos Krampis Guillermo I. Perez-Perez Kelly V. Ruggles Christina Schweikert Steven Shen Phillip Ross Smith Zuojian Tang Jinhua Wang

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